What is the region of dna where rna synthesis begins?

The region of dna where rna synthesis begins _________ is the region where the transcription of mRNA is initiated by initiation proteins that are not in the polymerase II enzyme. It begins on the minus strand with a small RNA molecule called tRNA-1Met, which is involved in transferring the amino acid methionine to the first protein on a polypeptide chain.


This region forms an initiation complex with an initiator tRNA and mRNA, and it forms a stalled ribosome that prevents both proteins from being translated until a pppGEM2C (5′ GTPase) binds to it, triggering rna synthesis. This activates translation, allowing both proteins to be translocated into their usual locations as well as allowing protein synthesis to recommence.


Some more facts:

It has been shown that the initiation complex can be reconstituted in vitro, completely independent of its location. This region is rapid and relatively stable, while other regions of dna are much more slowly moving.  It is also distinct from other regions in that it contains only two major regions: the upstream portion (containing tRNA and initiating protein) and the downstream portion (containing tRNA and mRNA). This makes it easier to distinguish between the active region and nearby folded regions. If DNA polymerase III (which is the last enzyme in a cell’s rna synthesis pathway) is inhibited by an inhibitor, translation ceases because no initiation complex is available for assembling a ribosome.


Important things:

It is important to note that the initiation complex is in fact a self-maintaining assembly, as an inactive tRNA-1Met or its initiator tRNA can still bind to its downstream partner, the ribosome. This prevents degradation until a sufficient amount of dna replication has occurred that the activity levels of some proteins are reduced enough that the complex can be produced again. The region of dna where rna synthesis begins is also referred to as the “decapping region”, because it is where termination occurs (or “decapsulates”). 


The region of dna where rna synthesis begins and its location on the chromosome were once debated, with two opposing theories as to how it works.  The Watson-Crick model held that the initiator tRNA docks into mRNA at point X (near the 3′ end) while it is still attached to the 5′ end of dna, and that termination happens when it moves to point Y near the 5′ end. This is an energetically favorable pathway, so it is possible that this model is correct.


What are the features?

The region of dna where rna synthesis begins contains two major features: a “decapping” site that is used for termination, and the initiation complex.


What is happening in the region?

The region of dna where rna synthesis begins contains a 5′ cap structure that is bound to the ribosome, which it helps to support. It also contains an initiator tRNA (AUG), whose function in initiation has been studied extensively. 


The initiator tRNA was thought to have a conformational change as it was translated, with its RNA chain unfurling ventrally while rotating around its own axis so that its anticodon loop faces upstream and its amino-acid-binding site faces downstream. This would promote its interaction with the initiator tRNA and reverse transcriptase, which then interacts with the 5′ cap structure and stimulates rna synthesis. 


This is the region that was once thought to contain a nucleosome. However, this structure is now thought to be a part of the structure itself and not a separate structure on top of it that helps facilitate transcription. 


Where do geneticists agree?

There are three regions where mutations can have functional consequences: these are the region of dna where rna synthesis begins, where transcription starts; and 2 other regions where mutations can have functional consequences: they are region 2 (5′ end of gene) and 1 (3′) in exon 3>2.


If you want to take your RNA synthesis one step further, then you can add over three million base pairs (see below), to do it on the other side of the same double strand. You can also see that this will require a change in the ratio of 5′ adenine, 2′ deoxyadenosine and 2′ thio-cytidine (all modified by methylation), and cysteine could also be involved. “For example, the addition of these 3 ‘base pairs’, where the oligo(dT) is on both strands, doesn’t increase homology.


What are the benefits?

The region of dna where rna synthesis begins has a very interesting role in the dna life cycle. It is basically involved in regulating gene expression and protein translation. The regulatory region for rna synthesis is generally considered to be the entire locus, because it is involved in regulating dna replication, transcription and translation.


Step back a bit, and you can see that genes are made of dna and are made up of gene loops, not just each loop. In fact, they have very little to do with individual exons. Each exon is one of the two noncoding regions between two coding regions (introns). The region where the ribosome binds to messenger rna is called a “capping site”, although it’s technically an “exon” that binds the ribosome.


What are the limitations?

The region of dna where rna synthesis begins may only be one piece of the puzzle to understanding how DNA and RNA are synthesized, but it is still an important piece.

“The point I’m trying to make is that, if you know how all these pieces fit together (as we clearly do not), then it becomes fairly trivial to fill in the rest.

There are many labels that could be given to describe me, but one thing’s for certain: I am an entrepreneur with passion. Whether it's building websites and social media campaigns for new businesses or traveling the world on business trips - being entrepreneurs means constantly looking at yourself in a different light so as not get bored of your own success!


Please enter your comment!
Please enter your name here